| #R001 | administrative | operational | mandatory | Great Lakes Guardians’ Council Meetings | aquatic life, other | The Minister shall ensure that at least one meeting of the Council is held before the first anniversary of the day subsection (1) comes into force and that at least one meeting is held in every subsequent calendar year. | Applies to the Great Lakes Guardians' Council | high |
| #R002 | administrative | operational | mandatory | Maintenance of Ontario’s Great Lakes Strategy | aquatic life, recreational water, other | The Minister shall maintain Ontario’s Great Lakes Strategy. | General ministerial obligation | high |
| #R003 | monitoring | reporting | mandatory | Monitoring and Reporting on Ecological Conditions | drinking water, aquatic life, recreational water | the Minister shall ensure that programs or other actions that will be used to monitor and report on the following ecological conditions of all or part of the Great Lakes-St. Lawrence River Basin and that are sufficient for the purposes of improving understanding and management of the Basin are established and maintained: 1. Harmful pollutants, including microplastics. 2. Water quality. 3. Impacts of climate change. 4. Hydrology. 5. Biological communities. 6. Such other conditions as may be prescribed by the regulations. | Except where existing programs are sufficient as per subsection (2) | high |
| #R004 | administrative | health | mandatory | Algae Blooms Target Establishment | drinking water, aquatic life, recreational water | Within two years after this section comes into force, the Minister shall establish at least one target under subsection (1) to assist in the reduction of algae blooms in all or part of the Great Lakes-St. Lawrence River Basin. | Mandatory target for algae reduction within two years of enactment | high |
| #R005 | administrative | operational | mandatory | Official Plan Conformity | other | The council of a municipality or a municipal planning authority that has jurisdiction in the area to which an initiative applies shall amend its official plan to conform with designated policies set out in the initiative, | Applies where a municipality or authority has jurisdiction in an initiative area | high |
| #R006 | administrative | operational | mandatory | Prescribed Instrument Conformity | other | a person or body that issued or otherwise created a prescribed instrument before an initiative took effect shall amend the instrument to conform with designated policies set out in the initiative. | Subject to regulations under clause 38 (1) (g), (h) or (i) | high |
| #R007 | operational | operational | mandatory | Compliance with Ministerial Directions | other | If the Minister of the Environment and Climate Change or the Minister of Natural Resources and Forestry directs a public body to do something under Part IV or V the public body shall comply with the direction. | When a direction is issued under Part IV (Targets) or Part V (Proposals for Initiatives) | high |
| #R008 | administrative | operational | mandatory | Review of Great Lakes Strategy | drinking water, aquatic life, recreational water, other | The Minister shall cause a review of the Strategy to be undertaken before December 17, 2018 and before every sixth anniversary thereafter. | Periodic review cycle | high |
| #R009 | reporting | reporting | mandatory | Triennial Progress Reporting | drinking water, aquatic life, recreational water, other | At least once every three years, the Minister shall, after consulting with the other Great Lakes ministers, prepare a report setting out the following: [requirements 1-9 in Section 8(1)] | Consultation with other Great Lakes ministers required | high |
| #R010 | administrative | operational | mandatory | Target Achievement Plans | drinking water, aquatic life, recreational water, other | With respect to a target the Minister of the Environment and Climate Change or the Minister of Natural Resources and Forestry has established under subsection (1) or (3), he or she shall, after consulting with the other Great Lakes ministers, prepare a plan setting out the actions that shall be taken to achieve the target. | Required once a target is established | high |
| #R011 | prohibition | operational | mandatory | Prohibition of Non-Conforming Municipal Actions | other | no municipality or municipal planning authority shall, (a) undertake, within the area to which an initiative applies, any public work, improvement of a structural nature or other undertaking that conflicts with a designated policy set out in the initiative; or (b) pass a by-law for any purpose that conflicts with a designated policy set out in the initiative. | Applies within initiative areas and for designated policies | high |
| #R012 | operational | operational | mandatory | Initiative Policy Implementation Compliance | other | If a public body is identified in an initiative as being responsible for the implementation of a policy described in Schedule 2 that is set out in the initiative, the public body shall comply with any obligations imposed on it by the policy. | Identification as responsible body in initiative | high |
| #R013 | administrative | reporting | mandatory | Mandatory Document Production for Enforcement | other | If an officer appointed under clause (1) (c) requests in writing that a person produce any documents or data that relate to an activity that is regulated or prohibited by a regulation made under clause (1) (a) or to a thing that a person is required to do by a regulation made under clause (1) (b), the person shall produce the documents or data for the officer within the time and in the manner specified by the officer in the request. | Upon written request by an authorized enforcement officer | high |
| #R014 | administrative | operational | mandatory | Consideration of Traditional Ecological Knowledge | other | If a First Nations or Métis community offers its traditional ecological knowledge to the Minister under subsection (1) for the purpose of assisting the Minister in carrying out any of the following activities, the Minister shall take into consideration the traditional ecological knowledge in carrying out the activity: [activities in Section 28(2)] | Applies if knowledge is offered by community | high |
| #R015 | administrative | operational | mandatory | Inter-Public Body Document Provision | other | If a public body that is responsible for one of the following matters requests that another public body provide copies of any documents or other records that are in its possession and control and that relate to the matter, the public body that receives the request shall comply with the request: 1. The development of a proposal for an initiative under Part V. 2. The development, amendment or review of an initiative under Part VI. 3. Reporting on the progress or implementation of an initiative. | Request must relate to Part V or VI initiatives or reporting | high |
| #R016 | administrative | operational | mandatory | Laboratory Licensing Requirement | drinking water | Under the Safe Drinking Water Act, 2002 (SDWA), only licensed laboratories located in Ontario and eligible out-of-province laboratories are allowed to conduct tests on samples of drinking water. | Applies to tests conducted on samples of Ontario drinking water | high |
| #R017 | administrative | operational | mandatory | ISO/IEC 17025 Accreditation Prerequisite | drinking water | As a prerequisite for licensing, laboratories and their testing methods must be accredited to the current International Organization for Standardization/International Electrotechnical Commission (ISO/IEC)17025 international standard General Requirements for the Competence of Testing and Calibration Laboratories. | Prerequisite for laboratory licensing | high |
| #R018 | administrative | operational | mandatory | Method Documentation and Validation | drinking water | Laboratory licensing requires that the laboratory properly document and validate its testing methods and that the laboratory demonstrate competence to perform the methods it employs. | | high |
| #R019 | reporting | reporting | mandatory | Immediate Processing and Reporting | drinking water | The method or supporting procedures must ensure that there are no delays in the immediate processing and reporting of results as required by regulations. | Microbiology tests must not include delayed or extended overnight refrigerated incubation | high |
| #R020 | reporting | reporting | mandatory | Reporting Overgrown Microbiology Tests | drinking water | The method must include a definition for overgrown “over-crowding, confluent or non-identifiable microbial growth” results so that overgrown results are reported as adverse test results where a standard exists. | | high |
| #R021 | administrative | operational | mandatory | Licence Amendment for Method Modifications | drinking water | The laboratory must receive a licence amendment application approval from the ministry prior to implementing the modified method. | Required for changes affecting original validation, including sample preservation, hold time, or QC requirements | high |
| #R022 | administrative | operational | mandatory | Controlled Document Management | drinking water | The method must be reviewed, approved for adequacy and issued by laboratory as a controlled document. | | high |
| #R023 | administrative | operational | mandatory | Method Identifier Requirements | drinking water | The method must include at a minimum a unique identifier indicate the current revision status date of issue revision number | | high |
| #R024 | administrative | reporting | mandatory | Authorized Method Submission Requirement | drinking water | The laboratory must include a copy of its authorized analytical method with the application. | | high |
| #R025 | administrative | reporting | mandatory | Deviation Documentation Requirement | drinking water | The laboratory must document deviations from the reference methods in its method. | Applies when laboratory methods deviate from the cited reference source | high |
| #R026 | operational | operational | mandatory | Calibration Standard Frequency | drinking water | The laboratory must prepare calibration standards as frequently as, or more frequently than, prescribed by the reference method. | | high |
| #R027 | administrative | operational | mandatory | Proficiency Testing Submission | drinking water | The applicant must demonstrate satisfactory proficiency testing by submitting the most recent set of results as part of the application. | A minimum of 1 set of successful proficiency tests is required | high |
| #R028 | monitoring | operational | mandatory | MDL Validation and Matrix Impact Analysis | drinking water | The laboratory must validate the MDL and determine any impact of the matrix effects from drinking water and any preservatives. | | high |
| #R029 | reporting | reporting | mandatory | Dioxins and Furans TEF Source Identification | drinking water | Laboratories must identify the source of the TEFs used for their calculations. | Applies when reporting toxic equivalent quantity (TEQ) for dioxins and furans | high |
| #R030 | administrative | operational | mandatory | Validation Data Submission Requirement | drinking water | Laboratories applying for licensing are required to include supporting validation data relating to the analyte’s specific performance requirements. | Required for licensing applications | high |
| #R031 | monitoring | operational | mandatory | Microbiology Daily Positive Control | drinking water | For microbiology methods, a suitable daily positive control must be included. | Specific to microbiology methods | high |
| #R032 | reporting | reporting | mandatory | Reporting Data Precision | drinking water | The laboratory’s test results reported must: be expressed in defensible significant figures [and] be expressed by 2 to 3 significant figures for the expression of chemical data include 1 additional digit more than the ODWQS concentration where possible. | | high |
| #R033 | operational | operational | mandatory | Analyst Precision Evaluation | drinking water | At a minimum, inter and intra analyst precision must be evaluated. | Applicable to ruggedness testing for alternate or in-house methods | high |
| #R034 | administrative | operational | mandatory | Test Volume Documentation Requirement | drinking water | The volume of the sample required for analysis (test volume) must be properly documented in the method and must be suitable for drinking water analysis. | | high |
| #R035 | operational | operational | mandatory | Linearity Validation Procedure | drinking water | To establish working linearity the laboratory must prepare, in triplicate, a minimum of 5 concentrations between upper and lower limit of the working range and analyze them. | Required for alternate reference or in-house methods | high |
| #R036 | operational | operational | mandatory | Confirmation of Identity Requirement | drinking water | The analytical technique must establish confirmation of identity. The laboratory must demonstrate that the response produced by the method is attributable to the analyte of interest. | Required for alternate or in-house methods | high |
| #R037 | prohibition | operational | mandatory | Microbiology Incubation Time Modification Prohibition | drinking water | For microbiology tests, the incubation time may not be modified. | Applies to modifications of microbiology reference methods | high |
| #R038 | operational | operational | mandatory | Hold Time Validation Stability Confirmation | drinking water | The hold time validation must include sufficient replicates and monitoring frequency of the analyte to confirm that the analyte is stable throughout the hold time. | Required when chemistry of extract is different or alternate preservatives are used | high |
| #R039 | administrative | operational | mandatory | Post-licensing Revision Documentation | drinking water | All revisions must be documented in the method and must be done in accordance with the terms and conditions in the laboratory’s drinking water testing licence. | Applies to optimization of analytical conditions after licensing | high |
| #R040 | operational | operational | recommended | MDL Calculation Methodology Guidance | drinking water | The laboratory should calculate the MDL in accordance with the laboratory’s policies and at a minimum it should follow the criteria in AWWA Method 1020B “Quality Control”. | | high |
| #R041 | operational | operational | mandatory | Alternate Method Sample Handling Consistency | drinking water | The proposed alternate methods must also have sample collection and handling procedures that are suitable for drinking water and consistent with an accepted test reference method listed in this Protocol. | Requirement for licensing of alternate or in-house methods | high |